Flow cytometry allows researchers to measure physical and chemical characteristics of thousands of cells per second as they pass single-file through a laser beam. It works a bit like a grocery store checkout scanner. Just as the scanner uses lasers to read barcodes and determine information about each grocery item, flow cytometry uses lasers to analyze individual cells suspended in fluid.
Cells first pass through a narrowed channel and align single-file before intersecting with one or more lasers. When hit with the laser, signals are generated that correspond to cell size, granularity, and fluorescent markers that were previously attached to proteins on the cell surface or inside the cell. These signals are picked up by detectors, processed by software, and generate quantitative data about each cell.
Researchers can attach fluorescent tags, like molecular barcodes, to cells using antibodies that bind to specific proteins. This allows different cell types to be distinguished based on their unique protein expression profiles. The resulting data provides high-resolution information on individual cells within complex or heterogeneous cell populations.
At the Stem Cell Medical Center, flow cytometry is used to thoroughly analyze and characterize stem cell samples prior to use in patient treatments. Their aim is to verify stem cell identity, quality, purity, and potency using this technology.
Specific applications of flow cytometry include:
The Stem Cell Center’s use of flow cytometry and adherence to strict quality control brings several advantages to patients:
In summary, the Stem Cell Medical Center’s use of advanced flow cytometry technology allows unparalleled analytical capabilities compared to microscopy or biochemical assays alone. This detailed stem cell screening prior to therapy provides patients confidence in the safety, consistency, potency, purity, and customization of their personalized stem cell treatments.
Contact us and schedule a consultation to see if you may benefit from regenerative treatments. Stem cell therapy could alleviate your pain and help you regain mobility.